Potassium tellurite agar is selective for the growth of staphylococci and is somewhat selective for the growth of corynebacteria. The cultures were classified as very sensitive inhibited by 0. Potassium tellurite is used together with agar as part of a selective medium for growth of some bacteria clauberg medium. Cool to 55c and add 50ml of laked horse blood sr0048 and 10ml of 3. Corynebacteria and some other species reduce teo 32 to elemental te, which stains the bacteria black. According to my observations, the potassium in the small petri dish reached equilibrium faster than its counter part in the waterfilled cylinder. A mineral with the formula teo 2 is called tellurite. The other one was in a tall cylinder filled with water. Technical information tellurite blood agar base product code. Serum tellurite agar, which contains lamb serum and potassium tellurite, was. Pdf role of potassium tellurite and brain heart infusion. Easy online ordering and nextday delivery available with 247 expert product support.
The complete medium is selective for staphylococcus aureus as the potassium tellurite inhibits most coliform organisms and is also reduced by staphylococcus aureus to tellurite giving typical black colonies on the baird parker medium. Formula adjusted, standardized to suit performance parameters. Blood agar is a type of growth medium trypticase soya agar enriched with 5% sheep blood that encourages the growth of bacteria, such as streptococci, that otherwise wouldnt grow. Cystine tellurite blood agar will appear hemolyzed due to the high tellurite content. The plasmid pdt64 has a high copy number and carries the tehab operon. One was done in a petri dish containing agar gel along with methylene blue. Potassium tellurite solution 1% safety data sheet date of issue 24082009. Full text full text is available as a scanned copy of the original print version. Sigmaaldrich potassium tellurite, 25g 45zv56p067725g. Selective media cystine tellurite blood agar or fresh tindales medium are. Blood agar is a generalpurpose enriched medium used to grow fastidious organisms and to differentiate bacteria based on their hemolytic properties. This products resides on a fisher scientific gsa or va contract. Links to pubmed are also available for selected references.
Tellurite blood agar base tm media titan biotech ltd. Tellurite blood agar is a selective medium used for isolation and cultivation of corynebacterium species 3,4. Thermo scientific remel middlebrook 7h9 broth with polysorbate 80 tellurite reduction medium is for the differentiation of mycobacterium spp. Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. It is cefixime potassium tellurite sorbitolmacconkey agar.
Egg yolk tellurite emulsion sterile, 20%, for microbiology find msds or sds, a coa, data sheets and more information. Why does potassium permanganate diffuse faster in agar gel. Hoyles agar is a selective medium that uses tellurite to differentially select corynebacterium diphtheriae from other upper respiratory tract flora. Composition ingredients gms litre casein enzymic hydrolysate 10.
A sterile egg yolk emulsion containing potassium tellurite for use in bairdparker medium base iso lab285 or bairdparker medium base lab085. Molecular weight of potassium tellurite convert units. Convert grams potassium tellurite to moles or moles potassium tellurite to grams. Potassium tellurite solution for use with modified giolitti and cantoni broth iso lab219 for the detection. If you are viewing this page as a nonregistered user, the prices displayed is list price. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Mechanism behind cystinetellurite blood agar essay sample. Serum tellurite agar is a selective and differential medium used for isolation of members of the genus corynebacterium, particularly in the laboratory diagnosis of diphtheria. Blood agar composition, principle, preparation and uses. Include trypticase soy agar with 5% sheep blood tsa ii plates as. In addition to possessing those prime virtues, simplicity and low cost, the medium is selective to a high degree due to the presence of potassium tellurite. The relative tolerance to potassium tellurite of various gramnegative bacilli, mostly of the colisalmonelladysenteryproteus fraternity, was tested in broth and on agar.
The addition of cystine greatly enhances the growth of c. A formulation of the new medium containing 5% glucose 5% sucrose, 0. Comparison of loefflers medium and tellurite blood agar in the examination of 302 nose. Nutrient agar with tellurite coagulase indicatioridentifies whether an organism produces the exoenzyme coagulase, which causes the fibrin of blood plasma to clotis selective or differential.
Pathogenic strains of corynebacteria can infect plants, animals, or humans. It is typically tryptic soy agar or columbia agar base with 5% sheep blood. These media contain specific amounts of nutrients but dont have trace of gelling agents such as gelatine or agar. Staphylococcus aureus is known to produce coagulase, not all staph bacteria do. Blood agar is an enriched, bacterial growth medium. Looking for sigmaaldrich potassium tellurite, 25g 45zv56. Tellurite blood agar base is used for the selective isolation and cultivation of. Potassium tellurite for microbiology catalog codes. Tellurite blood agar is a selective medium used for isolation and cultivation of corynebacterium species 1, 2. The medium appears cream to yellow colored, and takes the form of a freefloating powder. Blood agar composition, preparation, uses and pictures. It crystallizes in an orthorhombic structure mm2 with no center of symmetry. The inhibitory action of potassium tellurite on coliform. The tinsdales agar base medium without serum and tellurite is stable indefinitely if stored in closed screwcapped tubes or bottles but after the addition of supplement serum and tellurite, the medium is stable only for 23 days if stored in the refrigerator.
General description potassium tellurite is a hygroscopic granular white powder. Search results for potassium tellurite at sigmaaldrich. Throat or nasal swab is directly inoculated and streaked on this agar medium. Lars gerhardsson, in handbook on the toxicology of metals third edition, 2007. Potassium tellurite k2teo3, tetrahedron reactivos analiticos, argentina. Tellurite blood agar base is used for selective isolation and cultivation. If material is being cultured directly from a swab, roll the swab over a small area. It is selective due to the presence of inhibitor and differential by means of ability of organism to reduce potassium tellurite.
A modification of neills medium for the isolation and differentiation of corynebacterium diphtheriae types. Potassium tellurite acts as a selective agent and has inhibitory activity against most grampositive and gramnegative bacteria except corynebacterium species. Get a printable copy pdf file of the complete article 921k, or click on a page image below to browse page by page. Cm0507 macconkey agar without salt cm0523 giolitticantoni broth. Cefixime potassium tellurite sorbitolmacconkey agar listed as ctsmac. Blood agar plates are enriched medium used to culture those bacteria or microbes that do not grow easily. Potassium tellurite at the concentration chosen inhibits gram negative and a variety of gram positive bacteria and allows detection of tellurite reduction which is typically but not exclusively found in corynebacteria. Blood contains inhibitors for certain bacteria such as neisseria and.
Other information msds first issued 20082002 msds data revised 24082009 revisions highlighted date of issue classification and labelling have been performed according to eu. They are typically colorless or white salts, which in some ways are comparable to sulfite. Cefixime potassium tellurite sorbitolmacconkey agar how. Nile chemicals,manufacturers, exporters, stains, ph indicators, chemicals, drugs, aluminium chloride, aluminum chloride, dyes, basic dyes, metal salts, phase. Although cryptic when present in cells as a single copy, cells with multiple copies of tehab convert potassium tellurite k 2 teo 3 to black metallic. Mechanism behind cystinetellurite blood agar essay example for free newyorkessays database with more than 65000 college essays for studying. Tellurite glycine agar base m448 tellurite glycine agar base is used for quantitative detection of coagulasepositive staphylococci from foods and other sources like skin, mucous membranes, faeces, air and soil. Staphylococci and corynebacteria reduce the potassium tellurite in the agar, which makes the colonies grow black. Media, a manual of products and procedures, fieskeville, ti. Bd tellurite agar hoyle approximate formula per liter purified water. Potassium is the seventh most abundant element on earth.
Answer these questions concerning the volume and composition of blood. List the solid elements of blood red blood cells, white blood. The number of electrons in each of potassiums shells is 2, 8, 8, 1 and its electron configuration is ar 4s 1. Broth medium serves various purposes such as propagation of large number of organisms, fermentation studies, and various other tests.
Use of a potassium tellurite medium in the detection of. Mechanism behind cystinetellurite blood agar essay 735. A tellurite compound, for example sodium tellurite, is a compound that contains this ion. This is a sterile emulsion of egg yolk in saline containing potassium tellurite and is generally used as a selective differential agent in baird parker medium. Streak the tinsdale agar plate to obtain wellisolated colonies. Corynebacterium diphtheriae on tellurite agar medical.
Application potassium tellurite along with agar may be used as a component of a selective medium for growth of bacteria. Biopeptone monopotassium phosphate or detecting faecal coliforms drinking in water waste water, seawater and foods samples by mpn method. Potassium tellurite k2teo3 inhibits the growth of gramnegative bacteria and most of the upper respiratory tract normal flora. Inoculate a tube of middlebrook 7h9 broth w summary and explanation in 1969, kilburn et al. Potassium tellurite k2teo3is used together with agar as part of a selective medium for growth of some bacteria. It crystallizes in an orthorhombic structure mm2 with no center of symmetry at. Glucosesucrosepotassium telluritebacitracin agar, an. The influence of potassium tellurite pt and brain heart infusion agar difco, two components of modified listeria selective agar medium lsamm, on the hemolytic phenotype of listeria spp. Macconkeys agar used for enterobacteriaceae members contains bile salt that inhibits most gram positive bacteria. Thermo scientific oxoid potassium tellurite 10 x 2ml. It is a highly nutritious medium generally used as a basal medium for preparing blood agar by supplementation with blood. Tellurite blood agar is a selective medium used for isolation and cultivation of corynebacterium species.
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